As they have a phagocytic function, removing pathogens and cell debris, macrophages usually contain abundant primary and secondary lysosomes. Neutrophils generally enter tissues in large numbers only in response to a disease stimulus. The fibroblast synthesizes the collagen and ground substance of the extracellular matrix. When we study smooth muscle and peripheral nerve tissue we will come back to this slide to try and distinguish between collagen fibers and fascicles of smooth muscle and/or nerve fibers and ganglia. Look for elongated nuclei, usually solitary, from which a modest amount of tapered cytoplasm extends from either one or both poles of the nucleus. Non-striated (involuntary) muscle E 5 Striated muscle, showing striations and nuclei LS 5 Adipose tissue, section 5 Pseudo-stratified ciliated: epithelium TS 5 Areolar tissue, stained for fibres and cells E 5 Spinal cord TS 5 Compact bone, for Haversian Canals and lamellae 5 Cardiac muscle showing the nuclei, striations and intercalated discs LS 5 Neutrophils can be identified by their granular cytoplasm and their multilobular, condensed nuclei. Collagenous fibers consist of types I, II, or III collagen and are present in all types of connective tissue. Hyaline Cartilage. Identify the structure that is enclosed by the brackets in this electron micrograph. 158-197, Ross and Pawlina (6th ed), Chapter 9 Adipose Tissue, pgs. slide 36 (Aorta, aldehyde fuchsin) View Virtual Slide. Histamine and heparin are found in the more "regular" looking granules (evenly dark and round). An excellent example of this is slide 40 from the trachea (part of the respiratory tract). "loose" versus "dense" connective tissue), sometimes it is not always possible to classify connective tissue in a given section; it may be a little loose, a little dense, a little fatty, etc. Look at the connective tissue in the submucosa which is the lighter staining area View Image between the intestinal epithelium and the smooth muscle layer. 65-81, Supporting/Connective tissues, Ross and Pawlina (6th ed), Chapter 6 Connective Tissue, pgs. Correct answer 3. Be sure you recognise favourable sections of the nucleas, mitochondria, and rough ER. If the closely packed bundles of fibers are located in one direction, it is called regular; if oriented in multiple directions, it is referred to as irregular. Ground substance is an aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular and fibrillar elements of the connective tissue. & Mass. Fibroblasts, adipocytes (fat cells), macrophages, and mast cells are … Note how the diameter of the fibers varies with location. Brown fat cells are highly specialized for temperature regulation. = acidophilic) region adjacent to the cell nucleus. Both are fluid, … Learn vocabulary, terms, and more with flashcards, games, and other study tools. Be able to use knowledge about the physical characteristics of collagen and elastin in explaining the functions of tissue where these molecules occur in large quantities (, Be able to recognize different types of connective tissue (. This region is also a loose, irregular connective tissue but can be so extensively infiltrated by white blood cells and plasma cells that the supporting fibers and ground substance are obscured. Mast cells are granulated cells typically found in connective tissue. Dense Connective Tissue. Dense connective tissue is divided into 1) dense regular, 2) dense irregular, 3) elastic. These two components will be the focus of this lab. You will encounter each of these later in the course; for now, make sure you recognize that they all descend from monocytes, and that the macrophage is the connective tissue version. You need to get 100% to score the 7 points available. Wheater's, pgs. This cartilage is found in the nose, tracheal rings, and where the ribs join the sternum. It is comprised primarily of type I collagen. Individual lacunae may contain multiple cells deriving from a common progenitor. Resists tension in one direction. Variations in the … The reticular fibers provide physical support for all the cells present in tissues subject to stretching. Note the uniform distribution of regularly arranged collagen fibers (type I). In these particular instances, don't worry so much about trying to exactly classify the tissue per se, but at least try to identify the cellular and extracelluar components that you can (also bear in mind that you can't always definitely identify every cell) and think how its overall appearance reflects its function. This slide has been stained with iron hematoxylin and eosin so you can see collagen fibers (orange) as well as elastic fibers (purple/black) in the dermis View Image. Usually there is more extracellular matrix than cellular material. The nuclei of many cells are not included in the field of view. Fibroblasts are by far the most common native cell type of connective tissue. In this photo of areolar connective tissue, nuclei of cells are stained but the cytoplasm is pale and not distinguishable. Dense regular connective tissue - the tissue shown has a dense, regular arrangement of collagen fibers; the cells present are fibroblasts NOT squamous epithelial cells. Macrophages are indistinguishable from fibroblasts, but can be recognized when they internalize large amounts of visible tracer substances like dyes or carbon particles. See more ideas about tissue, things under a microscope, fun facts. Most frequently, the different types of connective tissues are specified by their content of three distinguishing types of extracellular fibers: collagenous fibers, elastic fibers, and reticular fibers. your own Pins on Pinterest This electron micrograph depicts mature fat cells. The cells of the connective tissue are formed from embryonic cells called mesenchymal cells. The very small cracks between the fibers are just artifacts of shrinkage that occured during tissue preparation. These tissues have two principal components, an extracellular matrix and a variety of support cells. Health Information Technology & Services. Many of the major types of cellular organelles are visible in this image. The basal lamina is anchored to the underlying connective tissue by fine fibrils oftype VII collagen (you obviously can't tell this looking at it in the light microscope, but you should recall this from lecture). They both have all three of the defining characteristics listed above, but their extracellular matrix is tougher, denser, and more solid than the various types of connective tissue proper. When sectioned material is observed with the light microscope, Dense Irregular Connective Tissue is often seen to contain small, thin, dark streaks that contrast with the surrounding pale-staining Collagenous Fibers. Discover (and save!) Organs are then formed by the functional grouping together of multiple tissues. Connective tissue is a type of animal tissue made up of cells, fibers, and gel-like substances that supports and gives structure to the body. Muscle tissue is specialized for contraction. In biology, tissue is a cellular organizational level between cells and a complete organ.A tissue is an ensemble of similar cells and their extracellular matrix from the same origin that together carry out a specific function. Fibroblasts are large, flat, elongated (spindle-shaped) cells possessing processes extending out from the ends of the cell body. ), so the elastic lamellae are purple. Download this Dense Connective Tissue In Microscope vector illustration now. With low power, locate the medulla (the interior) of the lymph node. Questions are provided for the most important slides of this chapter. The extracellular matrix is made up of fibres in a protein and polysaccharide matrix, secreted and organised by cells in the extracellular matrix. Brown fat is important for thermoregulation in newborns and hibernating mammals. In this area note the irregular, wavy collagen fibers arranged singly or in small groups. Connective tissue is divided into four main categories: Connective proper; Cartilage; Bone; Blood; Connective tissue proper has two subclasses: loose and dense. associated with allergies and other type I reactions. The tissue sections after fixation and wax embedding are typically cut into two to five micron thin slices with a microtome before staining and transfer to a glass slide for examination with a light microscope. In this area note the irregular, wavy collagen fibers arranged singly or in small groups. Observe the branching nature of the elastic fiber and the "mantle" of elastic microfibrils. As fibroblasts are the most common cells in areolar tissue, the majority of the nuclei seen here are probably fibroblast nuclei. As you look at this section, you will see a very obvious layer of mucous epithelial cells (PAS also reacts with the carbohydrate-rich mucin). 7 Types of Connective Tissue - Microscope Slides learn by taking a quiz; Online quiz to learn 7 Types of Connective Tissue - Microscope Slides; Your Skills & Rank. Overview of Connective Tissue. Look for adipose tissue in Slide 30 which is taken from abdominal mesentery (the connective tissue that suspends the viscera within the abdominal cavity). They are especially common along smaller blood vessels. The granules are often so dark that they obscure the nucleus. Make sure you can see the difference between cross sectioned and longitudinally sectioned collagenous fibrils. 63 Macrophage View Virtual EM Slide At high magnification, the larger strands are seen to be made up of bundles of smaller fibres. 21 Plasma cell View Virtual EM Slide Be sure you look at both the H&E and Masson trichrome-stained slides as they provide an excellent opportunity to see how collagen stains in connective tissue when either stain is applied. Slide 27 (lymph node, H&E) View Virtual Slide, Slide 28 (lymph node, silver stain) View Virtual Slide. Elastic cartilage is characterized by the presence of abundant elastic fibers and is quite cellular. They release histamine during allergic reactions. Finally, recall that PROcollagen is INTRAcellular and doesn't aggregate into fibrils as shown here until secreted. The function of white fat is to serve as an energy source and thermal insulator. 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