• When evaluating “Sex as a biological variable…”, the Principal Investigator (PI) and ACUC must consider age since sex is undetermined until at least 25 dpf.10,11 Common Zebrafish Procedures: Anna Ghilardi, Alberto Diana, Laura Prosperi, Luca Del Giacco, Expression pattern of the small muscle protein, X-linked (smpx) gene during zebrafish embryonic and larval developmental stages, Gene Expression Patterns, 10.1016/j.gep.2020.119110, (119110), (2020). In cardiovascular research, it has been used to model specific cardiac phenomena as well as to identify novel therapies for human cardiovascular disease. [49] [50] In 2011, the British Heart Foundation ran an advertising campaign publicising its intention to study the applicability of this ability to humans, stating that it aimed to raise £50 million in research funding. With the powerful zebrafish model, this study intends to clarify the effects of host and environment on the gut microbiota succession across fish developmental stages (from larvae to adults) by manipulating zebrafish hatching and rearing environments. After washing the drug, we recorded and analyzed the first 5 and 30 min of the motor behavior of 5, 7, and 14 dpf subjects. AFB1 belongs to the family of aflatoxins and is one of the mycotoxins highly present in fish feed worldwide. The zebrafish transcriptome during early development "The three earliest developmental stages were similar when comparing highly expressed genes, whereas the 50% epiboly stage differed from the other three stages in the identity of highly expressed genes, number of uniquely expressed genes and enrichment of GO molecular functions. Zebrafish pigment variants Albino fish and casper fish have been genetically modified to remove some or all of their skin pigmentation, giving researchers a clearer view of their brain. Zebrafish larvae inflate their swim bladder and become free swimming between ∼96 and 144 h postfertilization (hpf). Zebrafish have the ability to regenerate their heart and lateral line hair cells during their larval stages. The Creator gave the zebrafish the ability to regenerate its skin, fins, heart, and even its brain in larval stages. Features . E xperimentation with zebrafish often requires manipulation and observation after larval hatching and through the first several days when larvae are expected to be free swimming and may be able to initiate feeding. The zebrafish (Danio rerio) is an increasingly popular animal model biological system. Aflatoxin B1. Two distinct experiments were conducted: a larval stage experiment (from 8 to 21 days post fertilization, dpf) and a juvenile stage … Early Larval Period 303 Acknowledgments 303 Glossary 303 References 309 INTRODUCTION Introduction. larval stages (up to 7 dpf).3-9 The number of larvae used may be provided as an estimate considering their size and normal housing conditions. 3.5. To test this hypothesis, we chronically treated zebrafish larvae from 3 to 5 dpf with DA. In this study, we set out to determine whether these behavioral alterations were sustained in larvae at older stages (7 and 14 dpf). In summary, CTN exerts cardiotoxicity effects in zebrafish larvae stages have been manifested as evidenced by alteration of different heart parameters (pericardial edema, heart looping, rate, …) and in gene expression. Therefore, this study aimed to improve the understanding of behavioural consistency across contexts and over time in zebrafish from the larval to juvenile stages. The larval zebrafish brain (shown in green) is made up of about 100,000 neurons and contains many similarities to the human brain. Zebrafish also seem to have the ability to regenerate photoreceptors and retinal neurons following injury.